Семинар по инновационным подходам в анализе поведения.
Организатор семинара: Компания TSE Systems GmbH Siemensstr. 21 61352 Bad Homburg Germany
Семинар состоится 12 апреля 2011 в конференцзале ИВНД и НФ РАН
(г. Москва ул. Бутлерова 5а, справки по т. (495) 3347000)
Программа Семинара
10:00 - 10:15 Открытие семинара. Проф. П.М.Балабан / Юрген Фемер (TSE
Systems)
10:15 - 11:15 Ингаляционная технология: нано и микрочастицы. Юрген Фемер, TSE Systems (на англ.)
11:15 - 11:45 Coffee break
11:45 - 13:00 Novel tools for phenotyping mice: Intellicage and Neurologger H.-P. Lipp (на англ., тезисы прилагаются)
13:30 - 14:00 Новые технологии и оборудование для in vivo исследований: обзор инновативных продуктов. Елена Венцлер ( TSE Systems) (на русск.)
13:00 - 14:00 Обсуждение и обед.
Тезисы
Novel tools for phenotyping mice: Intellicage and Neurologger. H.-P. Lipp Institute of Anatomy, University of Zurich, Switzerland
Behavioral phenotyping of mutant mice faces several challenges due to the steadily increasing number of genetically modified mice. For one, the number of animals awaiting behavioral testing exceeds the capacities of standard behavioral laboratories. Secondly, many of the commonly used behavioral tests reveal high variability not only between animals but, more disturbingly, between laboratories. Thirdly, mutant mice often show pleiotropic phenotypes with variable penetrance of particular subtypes, whcih requires extensive testing. Finally, behavioral tests depend on measuring movements or suppression thereof, yet it is not always clear whether changes in movement are truly associated with memory – a prime example being contextual fear conditioning. Having realized these problems during testing a vast number of mutations, we sought for methods that could provide more efficient and more standardized methods for behavioral testing. During field studies in Russia, we realized that gene and treatment effects on behavior can be tested in social groups over prolonged time without human supervision using transponder-tagged animals. Thus, we developed IntelliCage, essentially a large home cage with 4 operant conditioning units, housing 12-16 mice undergoing a variety of tests involving various forms of spatial learning and operant conditioning schedules reward or punishment as reinforcement. All tests are programmable and run without human handling (a major source of variability in standard tests). The system has been used sucessfully by different laboratories for diverse mutant mice. In our laboratory, David Wolfer focused primarily on validating the system by analyzing the behavior of mice with complete bilateral hippocampal lesions both in standard laboratory tests and in IntelliCage. IntelliCage recognizes such hippocampal lesions in a variety of tests. For example, mice showed severe impairment of reversal learning in both the water maze and in Intellicage. On the other hand, the Intellicage system also revealed a number of deficits that are generally not analyzed in standard tasks or have moved out of focus: lesioned mice show spatial stereotypies, reactive hyperactivity after every change of the task or situation, and are impaired in precise timing of search activities. Presently, we are developing Intellicage prpograms for measuring impulsivity, and develop add-ons (IntelliMaze) for testing social memory and interactions. One less apparent feature ist the ability of IntelliCage to measure exploration and circadian activity. The neurologger system, essentially a very small data logger, was initially designed for recording neuronal activity and EEG from flying homing pigeons. Its adaptation to mice envisioned easy screening for epilepsy, and recordings during tasks such as fear conditioning (in order to detect whether moving mice show fear responses) or EEG activity during social interactions.
Taken together, these two systems can almost replace a laboratory analyzing behavior and electrophysiology of mice, particularly so if the task of the laboratory is to employ standardized defined tests. Yet they are also helpful for in-depth analysis of behavior particularly if this requires prolonged testing. In our laboratory, both systems became indispensable and IntelliCage is in fact slowly replacing most other behavioral tests.